Development of Eucalyptus tissue culture conditions for improved in vitro plant health and transformability
نویسندگان
چکیده
Background Despite its importance as a widely-planted crop tree, eucalypt species and hybrids are relatively difficult to micropropagate, culture and genetically transform in vitro. Compared to other plant species, few non-commercial laboratories are proficient at Eucalyptus tissue culture and transformation. We have undertaken to establish and transform several eucalypt clones in the laboratory. Our main aims include the identification of clones amenable to culturing and transformation, and the development of robust and transferable micropropagation, organogenesis and transformation protocols to enable routine production of transgenic eucalypts for public sector research. Efficient transformation protocols are essential to take full value of the eucalypt genome for functional genomics, ecophysiology, and biotechnology.
منابع مشابه
I-16: Assessment of The Vitrified Ovarian Tissue in Long Term Culture
In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...
متن کاملProduction of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC
To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...
متن کاملProduction of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC
To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...
متن کاملPlant tissue culture studies in Sorghum bicolor: immature embryo explants as the source material
Sorghum is a wonder crop from physiological point of view. It is the most important cereal crop, after rice and wheat. The number of reports describing the use of transgenic Sorghum for basic studies in Biotechnology is still limited when compared to other crops. In one hand, the success of the transformation techniques is mainly dependent upon the availability of optimal protocols for highly ...
متن کاملI-6: Follicle Development in Culture of Frozen / Thawed Human Ovarian Tissue
The primary therapeutic goal for the oncology patient is survival. Recent advances in diagnoses and treatment of neoplasia have resulted in an ever-increasing number of patients being cured and resuming a normal life. However, recognized side effects of treatments used to eradicate malignancies are temporary or permanent sterility. The cryopreservation of ovarian tissue harvested before cancer ...
متن کامل